Alkyl Hydroperoxide Reductase Subunit C (AhpC) Protects Bacterial and Human Cells against Reactive Nitrogen Intermediates
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چکیده
منابع مشابه
Roles of alkyl hydroperoxide reductase subunit C (AhpC) in viable but nonculturable Vibrio parahaemolyticus.
Alkyl hydroperoxide reductase subunit C (AhpC) is the catalytic subunit responsible for the detoxification of reactive oxygen species that form in bacterial cells or are derived from the host; thus, AhpC facilitates the survival of pathogenic bacteria under environmental stresses or during infection. This study investigates the role of AhpC in the induction and maintenance of a viable but noncu...
متن کاملActivity of Alkyl Hydroperoxide Reductase Subunit C 1 and C 2 of Vibrio 1 parahaemolyticus against Different Peroxides
Activity of Alkyl Hydroperoxide Reductase Subunit C1 and C2 of Vibrio 1 parahaemolyticus against Different Peroxides 2 3 Chun-Hui Chung, Tsung-yong Ma, Shin-yuan Fen, and Hin-chung 4 Wong* 5 6 Department of Microbiology, Soochow University, Taipei, Taiwan 111, 7 Republic of China 8 9 Running title: AhpC of Vibrio parahaemolyticus 10
متن کاملSupplemental Data: Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase
METHODS Spectral and Anaerobic Measurements. All absorbance spectra and anaerobic titrations employed the thermostatted Milton Roy Spectronic 3000 diode array spectrophotometer. The oxidoreductase and DTNB assays were performed on a thermostatted Applied Photophysics DX.17MV stopped-flow spectrofluorometer. The molar extinction coefficients of the proteinbound FAD at 448 or 449 nm were determin...
متن کاملCysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase system.
AhpC and AhpF from Salmonella typhimurium undergo a series of electron transfers to catalyze the pyridine nucleotide-dependent reduction of hydroperoxide substrates. AhpC, the peroxide-reducing (peroxiredoxin) component of this alkyl hydroperoxidase system, is an important scavenger of endogenous hydrogen peroxide in bacteria and acts through a reactive, peroxidatic cysteine, Cys46, and a secon...
متن کاملNADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly
The NADH oxidase-peroxiredoxin (Prx) system of Amphibacillus xylanus reduces hydroperoxides with the highest turnover rate among the known hydroperoxide-scavenging enzymes. The high electron transfer rate suggests that there exists close interaction between NADH oxidase and Prx. Variant enzyme experiments indicated that the electrons from β-NADH passed through the secondary disulfide, Cys128-Cy...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 1998
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80079-9